57 US AI Startups That Raised $100M or More in 2025

2025 has matched last year’s record pace for mega AI funding rounds. Here are the 49 US AI startups that secured $100M or more this year, month by month.

57 US AI startups that raised $100M or more in 2025. The AI boom is accelerating faster than ever. After a record-breaking 2024, the momentum has continued into 2025 with 57 US AI startups raising $100 million or more. This includes major infrastructure players, AI agent companies, coding assistants, enterprise tools, medical AI, and several newly minted billion-dollar labs.

Not only has 2025 matched last year’s count, but more companies have raised multiple mega-rounds, and several deals exceeded the $1B mark, showing investor confidence is still strong in frontier AI development.

Below is a full breakdown of every US AI company that raised a $100M+ round in 2025, organized by month.

US AI Companies That Raised $100M or More in 2025

November 2025

1. Anysphere – $2.3B

Valuation: $29.3B
Raised its second mega-round this year, powered by demand for its viral AI coding agent, Cursor.

2. Parallel – $100M Series A

Co-led by Index Ventures and Kleiner Perkins; building infrastructure for AI agents.

3. Hippocratic AI – $126M Series C

Healthcare AI agent platform; second raise of 2025.

October 2025

4. Fireworks AI – $250M Series C

Helps developers build AI apps using open-source models.

5. Uniphore – $260M Series F

Enterprise AI startup valued at $2.5B.

6. Sesame – $250M Series B

A fast-growing voice AI company.

7. OpenEvidence – $200M Series C

Medical AI chatbot startup; second raise this year.

8. Lila Sciences – $350M Series A

Building an AI-driven science superintelligence platform.

9. Reflection AI – $2B Series B

DeepSeek competitor backed by Nvidia.

10. EvenUp – $150M Series E

AI for the personal-injury legal industry.

57 US AI startups that raised $100M or more in 2025 — US AI startups that raised $100M or more in 2025

September 2025

11. Periodic Labs – $300M seed

AI scientist platform backed by Felicis and a16z.

12. Cerebras Systems – $1.1B Series G

AI infrastructure giant valued at $8.1B.

13. Modular – $250M round

AI infrastructure and tooling provider.

14. Distyl AI – $175M Series B

Enterprise AI workflow automation.

15. Upscale AI – $100M seed

AI infrastructure startup with notable investor lineup.

16. Groq – $750M Series E

AI inference acceleration company.

17. Invisible Technologies – $100M fundraise

AI training startup now valued at $2B.

18. Cognition AI – $400M Series C

Makers of Devin, the AI coding agent.

19. Baseten – $150M Series D

Production infrastructure for AI developers.

20. Sierra – $350M round

Bret Taylor’s customer service AI agent startup.

21. You.com – $100M Series C

Personalized AI search engine.

22. Anthropic – $13B Series F

Now valued at $183B; second raise of the year.

August 2025

23. EliseAI – $250M Series E

AI for healthcare and housing automation.

24. Decart – $100M round

AI research lab valued at $3.1B.

Related:

July 2025

25. Fal – $125M Series C

Generative media platform.

26. Ambience Healthcare – $243M Series C

AI healthcare operating system.

27. Reka AI – $110M Series B

Valued at $1B.

28. Thinking Machines Lab – $2B seed

Massive early-stage raise for AI research.

29. OpenEvidence – $210M Series B

Second appearance in this year’s list.

30. Harmonic – $100M Series B

Mathematical reasoning engine.

June 2025

31. Abridge – $300M Series E

Healthcare AI unicorn.

32. Harvey – $300M Series E

AI for legal operations.

33. Tennr – $101M Series C

AI-first healthcare automation.

34. Glean – $150M Series F

AI enterprise search player.

35. Anysphere – $900M Series C

First of its two 2025 mega-rounds.

May 2025

36. Snorkel AI – $100M Series D

AI data-labeling infrastructure.

37. LMArena – $100M seed

Community-driven AI model benchmark.

38. TensorWave – $100M Series A

AI infrastructure startup.

April 2025

39. SandboxAQ – $450M Series E

Quantum + AI model company.

40. Runway – $308M Series D

Creator-focused generative media AI.

March 2025

41. OpenAI – $40B round

Record-breaking valuation of $300B.

42. Nexthop AI – $110M Series A

AI infrastructure firm.

43. Insilico Medicine – $110M Series E

Generative AI for drug discovery.

44. Celestial AI – $250M Series C

Optical interconnect AI systems.

45. Lila Sciences – $200M seed

Early round prior to October raise.

46. Reflection AI – $130M Series A

Autonomous systems AI.

47. Turing – $111M Series E

AI coding tools and integrations.

48. Shield AI – $240M Series F

Defense AI technology.

49. Anthropic – $3.5B Series E

Massive early-2025 raise.

February 2025

50. Together AI – $305M Series B

Open-source generative AI stack.

51. Lambda – $480M Series D

AI compute and infrastructure.

52. Abridge – $250M Series D

First of two 2025 raises.

53. Eudia – $105M Series A

AI legal tech platform.

54. EnCharge AI – $100M Series B

AI hardware and chips.

55. Harvey – $300M Series D

Early-year raise prior to June round.

January 2025

56. ElevenLabs – $180M Series C

Synthetic voice platform valued at $3B.

57. Hippocratic AI – $141M Series B

Healthcare-focused LLMs.

2025 has become one of the most active years ever for AI investment. With 57 US startups raising $100M or more, the US continues to dominate global AI development. Mega-rounds, multi-round funding streaks, and skyrocketing valuations show how competitive the AI race has become, especially in infrastructure, autonomous systems, healthcare, and coding tools.

As the year closes, it’s clear that the next wave of AI giants is already being built.

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